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Arsenal were beaten by Southampton at the weekend to end a 22-match unbeaten streak in all competitions.
Unai Emery’s men now host north London rivals Tottenham in the Carabao Cup tomorrow (7.45pm) as they look to get back on the winning trail.
Arsenal’s defence was criticised for the 3-2 loss to Southampton while Leno made an error for Charlie Austin’s winner.
And the German is now fully focused on looking ahead rather than backwards.
“It was not easy defensively because this line of defence didn’t play together before,” Leno said.
“We have a big squad so we have many players who can play in defence or at centre back, so hopefully it won’t be a problem.
“I hope there will be no more injuries.
“After 22 games you can lose a game, so it is disappointing but now nobody talks anymore about the unbeaten run and we can look forward.
“We have Spurs on Wednesday – another big game – and maybe now we can start another unbeaten run.”
Arsenal’s long unbeaten run has come in Emery’s first season as Gunners manager.
He suffered defeat to Chelsea and Manchester City early in the season but was then unbeaten since August.
But pundit Tony Cascarino believes the loss and end of the sequence may not be such a negative.
“The defeat by Southampton was probably Arsenal’s worst display under Unai Emery but that is no bad thing in some respects,” Cascarino wrote for The Times.
“They have had a great run and are showing some really positive signs.
“But it will take a season for Emery to get the side how he wants it and results such as losing away to a team in the bottom half will happen.
“I know he was missing three senior centre backs at but it is still an area for the club to strengthen.”